Process for the Preparation of Immunogens or Diagnostic Reagents, and Immunogens or Diagnostic Reagents Thereby Obtainable

摘要

A process for the preparation of immunogens or diagnostic reagents that mimi c an antigen or a pathogenic organism specific to a disease, essentially characterized by the following operations: identificat ion of at least one antibody that reacts with the antige n or pathogenic organism specific to the disease; construction of phage libraries which disp lay on the surface of the capsid oligopeptides, expresse d from random sequence oligonucleotidic inserts introduced into a gene coding for a phage capsid protein using genetic manipulation techniq ues (for example using a plasmid engineered for the purposes of the invention, t he genetic map of which is shown in the figure); selecti on of the phages that display on the surfaces of the capsid antigenic oligopept ides recognized by said antibody; optional use of the se lected phages and/or fragments thereof and/or their derivatives for the formulation of diagnostic kits for the specific pathogenic agent, o r in general for the diseases, including immunological disorders typical of so-called aut oimmune diseases, with known or unknown etiology and/or pathogenesis; optional use of the selected phages and/or fragments thereof a nd/or their derivatives for the formulation of an antago nist of the antigen-antibody reactions for treatment of the disease induced by said antigen; optional use of the selected phages and/or frag ments thereof and/or their derivatives to induce a tolerance of the phenomena of h ypersensitivity and/or allergy to compounds and/or natur al or synthetic preparations; optional immunization of an organism by means of the selected phages and/or fragments thereof and/or thei r derivatives; and optional verification of the presence, in the serum of the immunized organism, of antibodies that recognize the abo ve antigen or organism specific to the disease.