Method for the determination of an analyte in a volume of a liquid sample, and its use for the determination of anti-TSH receptor autoantibodies in a patient's serum

摘要

Method for the determination of an analyte in a volume of a liquid sample, in particular of anti-TSH receptor autoantibodies in a patient's serum, in which the sample is mixed, to form a liquid reaction mixture, with one or more determination reagent(s) which contains or contain a) a predetermined amount of a binder (B) for the analyte (A), b) a predetermined amount of a competitor (K) which is likewise bound by the binder (B), in particular in such a way that the extent of its binding to the binder (B) correlates with the simultaneous presence of the analyte (A) and/or the amount thereof in the sample, where the competitor (K) is or can be labelled with a label which permits its qualitative and/or quantitative determination, where c) the liquid reaction mixture is simultaneously or sequentially reacted with a solid phase to which a substance (Imm) for the selective immobilisation of the amount, which is not bound to the binder (B), of the competitor (K) is bound, plus, where appropriate, a labelled reactant (Mark) for the immobilised competitor (K) and, after quantitative separation of the solid phase from the liquid reaction mixture, the amount of competitor (K) which is bound to the solid phase is determined in a manner known per se on the basis of a physical and/or chemical detection of the label which is bound to the latter, and the determined amount of competitor (K) ... Original abstract incomplete. <IMAGE>