发明名称 |
PROCESS FOR THE PREPARATION OF IMMUNOGENS OR DIAGNOSTIC REAGENTS, AND IMMUNOGENS OR DIAGNOSTIC REAGENTS THEREBY OBTAINABLE |
摘要 |
A process for the preparation of immunogens or diagnostic reagents that mimic an antigen or a pathogenic organism specific to a disease, essentially characterized by the following operations: identification of at least one antibody that reacts with the antigen or pathogenic organism specific to the disease; construction of phage libraries which display on the surface of the capsid oligopeptides, expressed from random sequence oligonucleotidic inserts introduced into a gene coding for a phage capsid protein using genetic manipulation techniques (for example using a plasmid engineered for the purposes of the invention, the genetic map of which is shown in the figure); selection of the phages that display on the surfaces of the capsid antigenic oligopeptides recognized by said antibody; optional use of the selected phages and/or fragments thereof and/or their derivatives for the formulation of diagnostic kits for the specific pathogenic agent, or in general for the diseases, including immunological disorders typical of so-called autoimmune diseases, with known or unknown etiology and/or pathogenesis; optional use of the selected phages and/or fragments thereof and/or their derivatives for the formulation of an antagonist of the antigen-antibody reactions for treatment of the disease induced by said antigen; optional use of the selected phages and/or fragments thereof and/or their derivatives to induce a tolerance of the phenomena of hypersensitivity and/or allergy to compounds and/or natural or synthetic preparations; optional immunization of an organism by means of the selected phages and/or fragments thereof and/or their derivatives; and optional verification of the presence, in the serum of the immunized organism, of antibodies that recognize the above antigen or organism specific to the disease. |
申请公布号 |
WO9426886(A2) |
申请公布日期 |
1994.11.24 |
申请号 |
WO1994IT00054 |
申请日期 |
1994.05.05 |
申请人 |
ISTITUTO DI RICERCHE DI BIOLOGIA MOLECOLARE P. ANG;FELICI, FRANCO;LUZZAGO, ALESSANDRA;NICOSIA, ALFREDO;MONACI, PAOLO;CORTESE, RICCARDO |
发明人 |
FELICI, FRANCO;LUZZAGO, ALESSANDRA;NICOSIA, ALFREDO;MONACI, PAOLO;CORTESE, RICCARDO |
分类号 |
G01N33/53;A61K35/76;A61K39/00;C07K7/08;C07K14/02;C07K14/18;C07K14/47;C07K16/32;C09K8/38;C12N7/00;C12N15/09;C12N15/10;C40B30/06;C40B40/02;G01N33/569;G01N33/576 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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