发明名称 |
Biotin-labelled DNA by polymerase chain reaction and detection thereof |
摘要 |
A format for hybridization capture of PCR amplified DNA on a solid support is provided. After labelling the amplified target DNA with biotin during amplification, the labelled DNA is specifically captured by base-pair hybridization to an amplicon-specific oligonucleotide capture probe which is bound to a solid support and the labeled DNA is detected with a biotin dependent chromogenic detection assay. In a specific format selected primers and probes are disclosed which enable the detection of Chlamydia trachomatis. |
申请公布号 |
AU7280494(A) |
申请公布日期 |
1994.11.24 |
申请号 |
AU19940072804 |
申请日期 |
1994.09.02 |
申请人 |
F. HOFFMANN-LA ROCHE AG |
发明人 |
MATHEW LONGIARU;SHERYL BETH SILVER;MICHAEL ANTHONY SULZINSKI |
分类号 |
C12M1/00;C07H21/04;C12N15/09;C12N15/11;C12Q1/48;C12Q1/68 |
主分类号 |
C12M1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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