NOVEL N-ALKYLACRIDAN CARBOXYL DERIVATIVES USEFUL FOR CHEMILUMINESCENT DETECTION

摘要

N-alkylacridan carboxylic acid derivative compounds (I) are used to generate chemiluminescence by the action of a peroxidase enzyme and an oxidant. The compounds (I) are useful in assays of all types. The figure is a graph showing a comparison of the light emission profiles from a series of five acridan compounds of the present invention. A 200 mu L volume of a reagent composition containing 0.1 mM acridan compound 1a-e in 0.1 M phosphate buffer, pH 8.9, 0.015 % (6.2 mM) hydrogen peroxide, 2.25 mM p-iodophenol, 0.5 % (/w) Tween 20 and 1 mM EDTA was reacted with 1 x 10<-13> mol of horseradish peroxidase.