| 摘要 |
PURPOSE:To obtain a novel DNA useful for creating a vector adapted to the expression of a gene in a basidiomycete. CONSTITUTION:The DNA has a base sequence expressed by the formula. A fragment of 1.2kbp (Hindlll-XbaI), the remaining ras gene and a fragment of 4.0kbp, cleaved with the restriction enzyme XbaI and containing the upstream side region (about 2.6kbp) are separated from a product obtained by cleaving the whole DNA of Lentinus edodes Sing. MS-LE1610 (FERM P-12960) with the restriction enzyme XbaI to afford the DNA of 2573 bp, expressed by the formula and containing the first exon (corresponding to 4 amino acids) in the whole base sequence thereof. |
