| 摘要 |
<p>A particle separator is provided for collection and manipulation of target particles, e.g., target cells, in a closed sterile field condition. In one embodiment, closed sterile field conditions are maintained from separation through concentration and/or cryo treatment steps and/or transfusion. Preservations of closed sterile field conditions are accommodated by using the same integrally coupled vessel for collection and concentration and transfer via integrally coupled conduits to a vessel for cryopreservation and/or transfusion. In one embodiment, a degree of cell concentration can be achieved, while maintaining closed field conditions, by using a hollow fiber device, preferably in-line between the column and the collection used. In another embodiment, a hollow fiber device is used in place of the column to achieve cell washing. In one embodiment, a plurality of valves are responsive to a data processor for controlling the path of fluid flow through the particle separator. A plurality of sensors are provided for providing sensor signals indicative of fluid flowing through the cell separator. A peristaltic pump is responsive to the microprocessor assembly for controlling the speed and direction of fluid flow through the system. A stir plate assembly is responsive to a signal from the data processor for controllably agitating the contents of the cell separator.</p> |
