发明名称 METHOD AND APPARATUS FOR LIQUID CHROMATOGRAPHY
摘要 In a method of filling a liquid chromatographic column with particulate separation medium, a liquid suspension of the particulate separation medium is introduced into one end of the column, wherein the column (1) is closed at one end with a first filter (5), so that the particles will be retained by the filter while the liquid passes therethrough. After terminating the filling process, the other end of the column (1) is closed with a second filter (12). According to the invention, the particle suspension is delivered via an inlet element (2) which can be moved axially in relation to the end of the column and in which the second filter (12) is mounted. During the filling process, the inlet element (2) and the filter (12) are held in a position in which the particle suspension is able to pass from the upper side of the filter (12) and past its side-edge and into the interior (14) of the column (1). After completion of the filling process, the inlet element (2) is moved towards the end of the column, while maintaining liquid pressure, to a position in which by-passage of the filter (12) is prevented, whereafer the inlet element (2) is fixed in this position. The invention also relates to a column for carrying out the method.
申请公布号 WO9425134(A1) 申请公布日期 1994.11.10
申请号 WO1994SE00362 申请日期 1994.04.25
申请人 PHARMACIA LKB BIOTECHNOLOGY AB;KARLSSON, KJELL;HELLSTROEM, KARL-GUNNAR 发明人 KARLSSON, KJELL;HELLSTROEM, KARL-GUNNAR
分类号 B01D15/00;G01N30/56;G01N30/60;(IPC1-7):B01D15/08 主分类号 B01D15/00
代理机构 代理人
主权项
地址