METHOD FOR SCREENING FOR TARGETS FOR ANTI-INFLAMMATORY OR ANTI-ALLERGIC AGENTS

摘要

This invention provides a method for identifying a cellular protein capable of specifically binding to an activated antibody receptor, whose cytoplasmic domain comprising an ARH1 motif, comprising (a) obtaining cells comprising receptors having the ARH1 motif; (b) lysing the cells under conditions whereby the native complex of the receptor having the ARH1 motif and the cellular protein is preserved; (c) isolating the complex; and (d) testing the associated receptor and the protein for biochemical activities, thereby identifiying the cellular protein capable of specifically binding to an activated antibody receptor, whose cytoplasmic domain comprising an ARH1 motif. This invention further provides a method for identifying a cellular molecule capable of being a target for designing drugs for autoimmune disease, inflammation or allergy which comprises (a) contacting a cell lysate with a molecule having a motif of amino acid sequence, AENTITYSLLKHP (SEQ ID NO:1) under the conditions permitting formation of a complex between the cellular target molecule with the motif; (b) isolating the complex formed in step (a); and (c) testing the complex for biochemical activities, thereby identifying the cellular molecule capable of being a target for designing drugs for autoimmune disease, inflammation or allergy.