| 摘要 |
This invention relates to a method for separating glycosylated proteins from non-glycosylated proteins. The method comprises the following steps: 1) equilibrating the protein containing solution with an equilibrating solution containing at least one organic solvent; 2) adding the equilibrated protein solution onto a chromatographic column containing a packing for the ion-exchange affinity separation of glycosylated and non-glycosylated proteins; 3) eluting the glycosylated proteins from the chromatographic column with a sufficient quantity of a first eluting solution containing at least one organic solvent and with or without at least one salt, wherein the salt concentration and/or organic solvent concentration and/or pH are appropriate for the eluting of glycosylated proteins being substantially free of non-glycosylated proteins; and 4) eluting the non-glycosylated proteins from the chromatographic column with a sufficient quantity of a second eluting solution containing at least one organic solvent and with or without at least one salt, wherein the salt concentration and/or organic solvent concentration and/or pH are appropriate for the eluting of non-glycosylated proteins being substantially free of glycosylated proteins. |
