发明名称 PROCESS AND DEVICE FOR PREPARING DNA SODIUM SALT
摘要 A process is disclosed for preparing desoxyribonucleic acid (DNA) from raw materials of animal origin. The raw materials of animal origin are crushed and homogenised in a nitrate salt solution, the homogenised material is treated at a high temperature with detergents and a concentrated sodium chloride solution, the reacted material is cooled, the cooled material is mixed with diatomite, the phases are separated by filtering and the end product is precipitated with ethyl alcohol. In order to improve the process so that it may be used on an industrial scale, ultrasound is applied for 10 to 80 minutes to the reacted material, before it is mixed with diatomite, the material being disposed in layers so that it may be uniformly and completely treated with ultrasound in predetermined conditions and at predetermined temperatures. The mixture ratio between the diatomite and the ultrasonically treated material equals 1:5 to 25 (weight in mg/volume in ml). The liquid phase separated by filtering is concentrated by pressure filtration and the precipitated DNA sodium salt is dried at 20 to 60 DEG C.
申请公布号 WO9425473(A1) 申请公布日期 1994.11.10
申请号 WO1994IB00054 申请日期 1994.02.28
申请人 EFTAG ENTSTAUBUNGS- UND FOERDERTECHNIK AG;WEINBERG, JURI PETROWITSCH;KAPLINA, ELLI NIKOLAJEWNA;KAPLIN, WALERIJ JURJEWITSCH;LADIGIN, ALEXANDER JEWGENJEWITSCH 发明人 WEINBERG, JURI PETROWITSCH;KAPLINA, ELLI NIKOLAJEWNA;KAPLIN, WALERIJ JURJEWITSCH;LADIGIN, ALEXANDER JEWGENJEWITSCH
分类号 C07H21/04;C12M3/00;C12N15/10;C12P19/34;(IPC1-7):C07H1/08 主分类号 C07H21/04
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