| 发明名称 |
Method for increasing the expression of polypeptides in recombinant cell culture. |
| 摘要 |
<p>Viable cell proportions and total cell densities in recombinant eukaryotic cell cultures are increased by transfecting a eukaryotic host cell with nucleic acid encoding a desired polypeptide and with an oncogene. Transfectant cultures are screened thereafter for the expression of elevated quantities of the desired polypeptide as compared to expression from the parent host cell.</p> |
| 申请公布号 |
EP0307248(B1) |
申请公布日期 |
1994.11.09 |
| 申请号 |
EP19880308387 |
申请日期 |
1988.09.12 |
| 申请人 |
GENENTECH, INC. |
发明人 |
LEVINSON, ARTHUR D.;MATHER, JENNIE P. |
| 分类号 |
C12N5/10;C07K14/62;C07K14/82;C12N9/64;C12N9/72;C12N9/88;C12N15/09;C12N15/67;C12N15/85;C12P21/00;C12Q1/04;C12Q1/68;(IPC1-7):C12N15/00;C12N5/00 |
主分类号 |
C12N5/10 |
| 代理机构 |
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