发明名称 |
Hybrid proteins between an extracytoplasmic enzyme and at least another protein, method for preparing them and also their applications |
摘要 |
The disclosed invention involves a DNA encoding a hybrid tripartite protein comprising an amino portion consisting of from 6 to 28 amino acid residues from the N-terminal of a mature E. coli alkaline phosphatase, a central portion corresponding to a heterologous polypeptide and a carboxyl portion which consists of the remainder of the mature E. coli alkaline phosphatase, a different alkaline phosphatase or an enzymatically active fragment of either. The tripartite proteins produced with this DNA can be used as a diagnostic reagent and in the screening of nucleic acid libraries or in the selection of recombinant clones.
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申请公布号 |
US5362644(A) |
申请公布日期 |
1994.11.08 |
申请号 |
US19920876910 |
申请日期 |
1992.04.30 |
申请人 |
COMMISSARIAT A L'ENERGIE ATOMIQUE |
发明人 |
BOQUET, PAUL;BOULAIN, JEAN-CLAUDE;DUCANCEL, FREDERIC;GILLET, DANIEL;MENEZ, ANDRE |
分类号 |
C12N9/00;C07H21/04;C07K14/005;C07K14/195;C07K14/46;C07K16/00;C07K19/00;C12N1/21;C12N9/16;C12N9/86;C12N15/09;C12N15/62;C12P21/00;C12R1/19;G01N33/535;(IPC1-7):C07K13/00 |
主分类号 |
C12N9/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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