Method of forming oligonucleotides

摘要

A process for non-enzymatic convalent ligation of two oligonucleotides having the structure shown below: The spontaneous reaction of these above oligonucleotides produces a thiophosphorylacetylamino linkage as shown below: When carried out in the presence of a template, this reaction gives 90% yields within 20 minutes at micromolar concentrations.