CELL FREE SYSTEM FOR PROTEIN SYNTHESIS AND USE OF CHAPERONE PROTEINS THEREIN

摘要

The present invention provides a novel high efficiency method for the cell free engineering and synthesis of protein. A novel method of the present invention comprises the steps of: preparing a cell free extract; separating out a ribosome fraction from said extract; incubating said ribosome fraction in the presence of a transcription/translation medium; and measuring the amount of protein synthesized. The method of the present invention may be used as a coupled transcription/translation system, a translation only system or a cell-free continuous flow system. Also provided are methods for synthesis of proteins and their correct folding using chaperone proteins.