FLUORESCENCE IMAGING WITH TUNABLE DETECTION

摘要

The present invention is a fluorescence imaging system comprising a light source (1) for irradiating an object (2), an optical filter (3) for separating fluorescence radiation emitted by said object and detector means (5) to generate a respective signal for each image point and a recording and computing unit for the recording of said fluorescence radiation in a plurality of wavelength bands and executing a mathematical and/or logic operation on signals on the same image point corresponding to one and the same point on the object, therewith to obtain an expression for a weighted signal value and to produce an image of the object from the weighted signal values for the different points of the object; said optical filter being tunable to an arbitrary wavelength passband within a large wavelength range. The optical filter is an electronically tunable interference filter.