PROTECTION OF HUMAN BONE MARROW FROM HIGH DOSE ANTIFOLATE THERAPY USING MUTATED HUMAN DIHYDROFOLATE REDUCTASE DNA

摘要

This invention provides a DNA vector which comprises DNA encoding a mutant, antifolate resistant, dihydrofolate reductase inserted into a site within the vector, the presence of which site is not essential for replication of the vector. This invention also provides bone marrow cells which comprise the above vector. Finally, this invention provides a method for reducing the toxic effects of antifolate therapy in a subject which comprises replacing the subject's hematopoietic cells with hematopoietic cells which comprise the above-described vector so as to reduce the toxic effects of antifolate therapy in the subject.