PRODUCTION OF RECOMBINANT FACTOR XA INHIBITOR OF LEECH HIRUDO MEDICINALIS

摘要

The present invention provides a recombinant polypeptide having the amino acid sequence X-tyr<26> - gly<110> where tyr<26> - gly<110> is identical to the sequence shown in Figure 10, and wherein X is methionine or absent and wherein asn<72> may be substituted by pro. The invention further provides a method of producing the polypeptide which comprises transforming a host cell with an expression plasmid encoding the polypeptide, culturing the transformed host cell so that the cell produces the polypeptide encoded by the plasmid, and a method of recovering the polypeptide so produced.