SELECTING GENES ENCODING CATALYTIC ANTIBODIES

摘要

The present invention describes methods of screening for or selecting a cata lytic antibody effective to cleave a target peptide. In particular, in the selection method of the present invention a phage gene is selected that encodes a gene product necessary for the production of a phage. The phage, carrying the modified gene is introduced into a host. Also, a library of rearranged immunoglobulin genes in a cloning vector is introduced into host cells. The host cells are grown under conditions in which the immunoglobulin genes are expres sed in the host cells. The presence of antibodies capable of cleaving the target peptide is identified on the basis of production of pha ge.