Method and device for determination of analytes in liquids.

摘要

Detection of an analyte (I) in liq. on the basis of a ligand test uses a sensor surface coated with a porous layer which is contcted with teh test liq. contg. (I) and one or more particulate agents (A) which have both signal-mediating and specific (I)-binding activities. The pores in the porous layer are no smaller than the diam. of (A) and allow passage of monomeric (A) to the sensor surface. (I) undergoes binding reaction with at least 2 (A) particles, forming a conjugate which, because of its size, cannot penetrate to the sensor surface. The sensor measures the amt. of (A) which has arrived at its surface from their signal-mediated properties. In another embodiment for analysis of particulate (I) in liq., (I) is bound to a specific binding agent immobilised on (or near) a sensor surface, and the liq. also contains at least one signal-mediating substd. (B). the approach of (B) to the sensor surface is limited by the bound (I) particles, so the immediate environment of the surface is altered in a way which can be detected by the sensor. Also new is appts. for these processes.