发明名称 |
Method of preparing fusion proteins |
摘要 |
Fusion proteins are obtained in high yields if a mixed oligonucleotide is constructed which codes for the ballast constituent of the fusion protein. The oligonucleotide mixture is introduced in a vector in such a manner that it is functionally linked to a regulatory region and to the structural gene for the desired protein. Appropriate host cells are transformed with the plasmid population obtained in this manner and the clones producing a high yield of coded fusion protein are selected.
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申请公布号 |
US5358857(A) |
申请公布日期 |
1994.10.25 |
申请号 |
US19930073508 |
申请日期 |
1993.06.09 |
申请人 |
THE GENERAL HOSPITAL CORP.;HOECHST AKTIENGESELLSCHAFT |
发明人 |
STENGELIN, SIEGFRIED;HABERMANN, PAUL;UHLMANN, EUGEN;SEED, BRIAN |
分类号 |
C07K14/62;C12N15/12;C12N15/62;(IPC1-7):C12N15/12 |
主分类号 |
C07K14/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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