发明名称 |
Gene expression in mammalian cells using a cap independent 5' noncoding region |
摘要 |
A construct which includes a cap independent 5' noncoding region of viral or cellular origin and a nucleotide sequence of interest, which is located downstream of the noncoding region. A method of producing a protein or a polypeptide of interest by introducing the construct, including a nucleotide sequence encoding the protein or the polypeptide of interest, into mammalian cells is also described. In one embodiment, the construct comprises all or a portion of the poliovirus cap independent 5' noncoding region and a nucleotide sequence encoding a protein or a polypeptide of interest.
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申请公布号 |
US5358856(A) |
申请公布日期 |
1994.10.25 |
申请号 |
US19920887223 |
申请日期 |
1992.05.18 |
申请人 |
WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH |
发明人 |
BALTIMORE, DAVID;TRONO, DIDIER |
分类号 |
C12N15/85;(IPC1-7):C12N5/10;C12P21/02 |
主分类号 |
C12N15/85 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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