| 摘要 |
An improved process for isolating A83543 factors from fermentation broth in which they are produced which comprises: a) adding an approximately equal volume of a water miscible, polar organic solvent to the fermentation broth, including the biomass thereof; b) separating the liquid phase of the resulting mixture from the biomass; c) adjusting the pH of the separated liquid phase to between about 7 and 13; d) applying the separated liquid phase directly to a column of nonfunctional, macroreticular polymer; e) eluting the A83543 components from the column with an aqueous solution of water miscible, polar organic solvent; and f) collecting the fractions containing A83543 components. |
