E1 PROTEIN GENE OF VARIANT PYRUVIC ACID DEHYDROGENASE COMPLEX AND E1 PROTEIN OF VARIANT PYRUVIC ACID DEHYDORGENASE COMPLEX

摘要

PURPOSE:To provide a new gene useful for the production of E1 protein of a variant pyruvic acid dehydrogenase complex. CONSTITUTION:The E1 protein gene of a variant pyruvic acid dehydrogenase complex has a function to code an amino acid sequence having a structure obtained by substituting the 146th arginine in the amino acid sequence of E1 protein of wild-type pyruvic acid dehydrogenase complex with proline. The objective gene has the base sequence of formula and is obtained form plasmid pACEE1K' DNA separated from E.coli JM109 (ferm BP-4486).