Biological support for cell cultures constituted by plasma proteins coagulated by thrombin, its use in keratinocytes culture, their recuperation and transport for therapeutic use.

摘要

Biological support for cell cultures, consisting of the coagulated mixture of a plasma protein concentrate and thrombin. The protein concentrate is obtained by precipitating fresh plasma with ethanol and contains equilibrated proportions of fibrinogen, Factor XIII and fibronectin. The thrombin concentration is adjusted to give the desired consistency of the coagulated carrier in the form of a film. The biological support is preferably used for the culture of keratinocytes, their recovery in the form of reconstituted tissue, and the transport of the latter. The reconstituted tissue is thus particularly suitable for use as a graft.