| 摘要 |
<p>A method and assay for the rapid, sensitive, accurate measurement of a component of the renin-angiotensin-aldosterone ('RAA') hormonal axis in a biological sample using spectrofluorometric measurement is disclosed. In the preferred embodiment a known quantity of a fluorescent labelled antibody immunoreactive with a specific RAA component is incubated with known volumes of the RAA component conjugated with an inert substrate and a biological sample. The amounts of bound and unbound fluorescent antibody are determined by spectrofluorometry, either after or during the incubation. The amount of RAA component in the sample is then quantified after construction of a standard curve based on spectrofluorometric measurements of fluorescent antibody bound to increasing amounts of RAA component in a second incubation mixture. As demonstrated by examples in which angiotensin is quantified, this method allows measurement of RAA components in picogram amounts in minutes and is useful for diagnosis of hypertension, renal disease, adrenal gland disease, and cardiovascular disorders.</p> |
