发明名称 |
ANALYTICAL TECHNIQUE FOR OLIGONUCLEOTIDE ANALOGS |
摘要 |
A method for separating a mixture of 5'-phosphorothioate mononucleotides, a mixture of 5'-phosphorothioate oligonucleotides, or a mixture of both 5'-phosphorothioate mononucleotides and 5'-phosphorothioate oligonucleotides comprising electro- phoresis through a gel of at least 12% polymerized linear acrylamide containing at least 50% formamide or other denaturing organic solvent. This method can resolve phosphorothioate oligonucleotides differing by only a single base. Samples of less than 1 nanogram in microliter volumes can be conveniently handled. |
申请公布号 |
WO9414824(A3) |
申请公布日期 |
1994.10.13 |
申请号 |
WO1993US12372 |
申请日期 |
1993.12.15 |
申请人 |
HYBRIDON, INC.;COHEN, AHARON, S.;VILENCHIK, MARIA |
发明人 |
COHEN, AHARON, S.;VILENCHIK, MARIA |
分类号 |
B01J20/26;C07H1/06;C07H19/06;C07H19/16;C07H21/00;C08L1/00;C08L1/26;C08L29/04;C08L33/24;C08L33/26;C12N15/10;G01N27/447 |
主分类号 |
B01J20/26 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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