| 摘要 |
<p>Method for the manufacture of an enzymatic sensor characterized in that it consists in implanting carboxyl or derivatives thereof-type covalent binding sites, by oxidation, on a substate consisting of at least one carbon thread comprising at least one carbon fibre and having a diameter less than or equal to 150 micrometers, preferably 100 νm and even more preferably, 50 νm; optionally de-oiling said fibre using an organic solvent; performing at least partial electrochemical oxidation, at a redox potential less than or equal to 3 volts with reference to a saturated calomel electrode (SCE), in an salt and/or aqueous solution, titrating a total of 0.1 to 2 M, preferably 0.2 to 1 M and even more preferably 0.3 to 0.5 M; and covalently grafting the enzymes on carboxyl or derivatives thereof-type sites, optionally after activation of the sites using a coupling agent. The invention also relates to enzymatic microsensors obtainable by carrying out said method.</p> |
