| 发明名称 |
REDUCED TEMPERATURE DNA DENATURATION USING GLYCEROL |
| 摘要 |
Method for determining the nucleotide base sequence of a double-stranded closed circular DNA. The method includes heating a solution which includes the DNA in the presence of at least 20 % glycerol and/or ethylene glycol to at least 80 DEG C to provide denatured DNA, and then sequencing the denatured DNA. |
| 申请公布号 |
WO9422798(A1) |
申请公布日期 |
1994.10.13 |
| 申请号 |
WO1994US03414 |
申请日期 |
1994.03.29 |
| 申请人 |
UNITED STATES BIOCHEMICAL CORPORATION |
发明人 |
FULLER, CARL, W.;PISA-WILLIAMSON, DENISE, A. |
| 分类号 |
G01N33/50;C07C31/18;C07C31/20;C12Q1/68;(IPC1-7):C07C31/20;C12P19/34;C07C31/22 |
主分类号 |
G01N33/50 |
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