METHODS AND DNA EXPRESSION SYSTEMS FOR OVER-EXPRESSION OF PROTEINS IN HOST CELLS

摘要

Disclosed are recombinant DNA vectors and methods for high levels of expression of proteins in bacterial host cells. The vectors contain a first gene encoding a protein product of interest, optionally as a fusion protein with ubiquitin, and a second gene encoding a cytoplasmic peptidyl-prolyl cis-trans isomerase. Co-expression of the two genes affords high levels of expression of the protein of interest, in some cases also increasing the degreee of solubility of the protein product.