发明名称 |
RNA Ribozyme which cleaves substrate RNA without formation of a convalent bond |
摘要 |
RNA enzymes or ribozymes can act as endoribonucleases, catalyzing the cleavage of RNA molecules with a sequence specificity of cleavage greater than that of known ribonucleases and approaching that of the DNA restriction endonucleases, thus serving as RNA sequence specific endoribonucleases. An example is a shortened form of the self-splicing ribosomal RNA intervening sequence of Tetrahymena (L-19 IVS RNA). Site-specific mutagenesis of the enzyme active site of the L-19 IVS RNA alters the substrate sequence specificity in a predictable manner, allowing a set of sequence-specific endoribonucleases to be synthesized. Varying conditions allow the ribozyme to act as a polymerase (nucleotidyltransferase), a dephosphorylase (acid phosphatase or phosphotransferase) or a sequence-specific endoribonuclease.
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申请公布号 |
US5354855(A) |
申请公布日期 |
1994.10.11 |
申请号 |
US19920843737 |
申请日期 |
1992.02.28 |
申请人 |
CECH, THOMAS R.;ZAUG, ARTHUR J.;BEEN, MICHAEL D. |
发明人 |
CECH, THOMAS R.;ZAUG, ARTHUR J.;BEEN, MICHAEL D. |
分类号 |
C12N9/12;C07H21/00;C12N9/16;C12N15/09;C12N15/10;C12N15/113;C12N15/54;C12R1/90;(IPC1-7):C12N15/11 |
主分类号 |
C12N9/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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