发明名称 Molecular clone of a chitinase gene from vibrio parahemolyticus
摘要 A process for cloning the chitinase gene of Vibrio parahemolyticus is provided, comprising the steps of cleaving the Vibrio parahemolyticus DNA with Sau3A, PSTI or other restriction enzyme, mixing the cleaved DNA fragments in the presence of pUC18 and T4 ligase to produce a composite plasmid, and inserting the composite plasmid in a DH5a strain of E. Coli.
申请公布号 US5352607(A) 申请公布日期 1994.10.04
申请号 US19920876894 申请日期 1992.04.29
申请人 LOUISIANA STATE UNIVERSITY AND AGRICULTURAL COLLEGE 发明人 LAINE, ROGER A.;OU, CHIN-YIH;JAYNES, JESSE M.
分类号 C12N1/21;C12N9/24;G01N33/569;(IPC1-7):C12N1/21;C12N15/31;C12N15/52;C12N15/70 主分类号 C12N1/21
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