| 摘要 |
<p>A DNA construct for use in treating a human carcinoma is disclosed. The construct includes a cancer-therapeutic gene under the control of a promoter and a group of enhancer sequences which are conserved in 5' flanking regions of the human epithelial cell secretory leukoprotease inhibitor gene and the human epithelial cell cytokeratin gene 8. Also disclosed is a method employing the construct to selectively kill carcinoma cells which are characterized by expression of epithelial cell secretory leukoprotease inhibitor protein in a transformed state, and a method for confirming effective gene delivery to and transcriptional activity in target epithelial cells.</p> |
