发明名称 |
IMPROVED ENHANCEMENT OF CHEMILUMINESCENT ASSAYS |
摘要 |
Chemiluminescent bioassays for the presence or concentration of an analyte in a sample use 1,2-dioxetanes as substrates for the enzyme of an enzyme complex that bind to the analyte. The chemiluminescence obtained from the decomposition of the dioxetane trigerred by the enzyme through the formation of the corresponding 1,2-dioxetane oxyanion of the enzyme complex is enhanced by the addition of TBQ as an enhancement agent. Other polymeric quatenary onium salts can be used as enhancements agents in conjunction with enhancement additives which improve the ability of the enhancement agent to form hydrophobic regions in the aqueous sample, in which regions the 1-2-dioxetane oxyanion and its chemiluminescent decomposition products can be sequestered. A kit for performing such assays is also provided.
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申请公布号 |
CA2157917(A1) |
申请公布日期 |
1994.09.29 |
申请号 |
CA19942157917 |
申请日期 |
1994.03.15 |
申请人 |
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发明人 |
BRONSTEIN, IRENA Y.;EDWARDS, BROOKS;VOYTA, JOHN C. |
分类号 |
G01N33/536;C07C43/178;C07C43/196;C07C69/00;C07D321/00;C07F9/12;C07F9/655;C07F9/6574;C07H15/203;C07H15/26;C12Q1/00;C12Q1/34;C12Q1/42;C12Q1/68;G01N21/76;G01N33/543;G01N33/58;(IPC1-7):C12Q1/42;G01N33/566;G01N33/569;C12Q1/70 |
主分类号 |
G01N33/536 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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