发明名称 |
Enzymatic membrane method for the synthesis and separation of peptides |
摘要 |
The present invention discloses a method for the enzymatic synthesis of a peptide. A protected peptide having a C-terminal carboxylate group or a protected, N-acyl amino acid having an alpha carboxylate group is reacted with a protected peptide having an N-terminal ammonium group or a protected amino acid having an alpha ammonium group in the presence of a condensation enzyme under conditions in which the carboxylate group and the ammonium group condense to form a protected, uncharged, peptide product. This peptide product is transported across a water-immiscible hydrophobic phase into an aqueous product phase and prevented from back diffusing across the water-immiscible hydrophobic phase. The peptide product can be converted, chemically or enzymatically, to a charged species that cannot back diffuse across the water-immiscible phase into the aqueous reaction phase. The water-immiscible hydrophobic phase is an ion rejection membrane separating the aqueous reaction phase from the product phase creating oil/water interfaces with each of the aqueous phases.
|
申请公布号 |
US5350681(A) |
申请公布日期 |
1994.09.27 |
申请号 |
US19920928673 |
申请日期 |
1992.08.12 |
申请人 |
THE COCA-COLA COMPANY |
发明人 |
IACOBUCCI, GUILLERMO A.;BROSE, DANIEL J.;RAY, RODERICK J.;VAN EIKEREN, PAUL |
分类号 |
C07K1/00;C07K5/072;C07K14/70;C12M1/40;C12P21/02;(IPC1-7):C12P21/00 |
主分类号 |
C07K1/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|