Multilayer analytical element for determination of total cholesterol in blood

摘要

In a dry type analytical element having at least one water-permeable layer including a porous spreading layer and at least one water-permeable layer, containing an enzyme having cholesterol esterase activity, the improvement which comprises containing a condensate of poly(ethylene oxide) monoalkylphenyl ether and formaldehyde at least in the water-permeable layer containing the enzyme or another water-permeable layer. In the analytical element of the invention, hemolysis does not occur and bound cholesterol is sufficiently decomposed to free cholesterol.