发明名称 |
Class I MHC-restricted T-T hybridomas, and a CD8-transfected BW5147, fusion partner therefor |
摘要 |
The present invention provides class I MHC-restricted T-T hybridomas, a novel T-T fusion partner therefor and an assay system that allows for the generation and detection of functional, class I MHC restricted T-T hybridomas. The fusion partner comprises a murine BW5147 cell line that has been transfected with a CD8 gene and stably expresses the CD8 gene product. The BW5147 transfectants are used to generate functional, class I MHC restricted T-T hybridomas which are permissive for lymphokine production. Functional class I MHC-restricted T-T hybridomas exhibiting the capability of producing lymphokines are prepared by fusing BW5147 cells expressing CD8 with T lymphocytes activated in a mixed leukocyte reaction (alloreactive T cells) or stimulated with antigen (antigen-stimulated T lymphocytes) and screening the resulting hybrids for the ability to produce lymphokines in response to appropriate antigenic stimulation. Responsiveness of these clones to alloantigen or to antigen-MHC I complexes can be tested by measuring their production of lymphokines, which provides a sensitive and quantitative assay system.
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申请公布号 |
US5348878(A) |
申请公布日期 |
1994.09.20 |
申请号 |
US19910814069 |
申请日期 |
1991.12.24 |
申请人 |
DANA-FARBER CANCER INSTITUTE |
发明人 |
ROCK, KENNETH L. |
分类号 |
C12N5/16;(IPC1-7):C12N5/16 |
主分类号 |
C12N5/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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