PRODUCTION OF AMINOACYL-AND MISAMINOACYL-TRNA

摘要

PURPOSE:To obtain an aminoacyl-tRNA or a misaminoacyl-tRNA useful for a protein synthesis system in vitro by reacting an amino acid (derivative) with tRNA in a specific condition. CONSTITUTION:A homologous amino acid (derivative) or a heterologous amino acid (derivative) is bound to tRNA by a reaction of the formula (a.a. is amino acid or amino acid derivative; a specific aminoacyl-tRNA hydrolase is aminoacyl-tRNA having no substrate specificity). It is preferable to use an aminoacyl-tRNA hydrolase derived from art emia larvae or fusariumculmorum.