A fluid sample receptacle provides a means of assuring uncontaminated, multiple samples of a fluid specimen. An upper or isolated sample chamber (10) and a lower or primary sample chamber (12) provide redundant test samples. A cover or lid (27) with a downwardly extending column (30) includes a stopper (34) with a removable plug (36) attached to automatically seal the lower sample chamber when the cover is sealed across the top of the upper chamber. This feature guarantees that the samples chambers are sealed or isolated simultaneously and that the test samples are therefore identical when taken. Tamper evident seals (17) prove that either sample has not been contaminated. A preferred embodiment provides a bellows assembly (90) to accommodate variations in ambient pressure and to provide a positive means of expelling a precise quantity of fluid from the receptacle for testing.