NOVEL L-SORBOSE DEHYDROGENASE AND NOVEL L-SORBOSONE DEHYDROGENASE OBTAINED FROM GLUCONOBACTER OXYDANS T-100

摘要

A novel L-sorbose dehydrogenase (SDH) and a novel L-sorbosone dehydrogenase (SNDH), both originating in gluconobacter oxydans T-100; a DNA coding for the SDH and/or the SNDH; an expression vector containing the DNA; a host cell transformed by the vector; and a process for producing the SDH and/or the SNDH by culturing the host cell in a medium. The invention SDH and SNDH are useful enzymes suitable for producing 2-keto-L-gulonic acid and further L-ascorbic acid. The invention process enables the SDH and SNDH to be mass-produced by genetic engineering techniques.