AN ENDOGENOUS TAXOL-LIKE SUBSTANCE IN HUMAN SERUM, MONOCLONAL ANTIBODIES DIRECTED THERETO, AND METHODS OF ASSAYING THEREFOR

摘要

The present invention provides two monoclonal antibodies capable of binding to taxol and taxol-like substances which are produced by hybridomas designated 69E4A8E, having ATCC Accession No. HB 11281 and 29B7B3C, having ATCC Accession No. HB 11280. The present invention also provides a method for detecting the presence of taxol or a taxol-like substance in a sample, a method for screening for a ligand in a subject which is not being treated with taxol, an endogenous taxol-like substance in human serum detected by this screening method, a method of quantitatively determining the amount of taxol or taxol-like substance in a biological fluid sample and a kit for assaying for taxol or a taxol-like substance in a sample. The present invention further provides an anti-idiotypic monoclonal antibody which mimics taxol. The invention provides an anti-idiotypic monoclonal antibody produced by hybridoma designated 82H11B9F, having ATCC Accession No. HB 11548. The present invention also provides methods of using the anti-idiotypic monoclonal antibody for determining the presence and amount of taxol or biologically active taxol derivatives and the presence and amount of receptors for taxol or biologically active taxol derivatives.