| 摘要 |
PURPOSE:To make possible to quickly and correctly perform diagnosis even in cold season without necessity of experience by mixing neutral red in a specific trichomonas-culturing medium. CONSTITUTION:0.001-0.01wt.% neutral red as an oxidation-reduction indicator is added to a medium containing A) 0.1-5wt.% nitrogen source (e.g. peptone, tryptose or casamino acid), B) 0.1-5wt.% carbon source (e.g. glucose), C) 8-20vol.% animal blood serum (preferably blood serum of horse or rabbit, etc.), D) 0.02-0.1wt.% agar-agar, E) 0.02-0.3wt.% reductive substance (e.g. L-cysteine hydrochloride or ascorbic acid) and F) 0.01-0.5wt.% growth-inhibitor against bacterium (e.g. antibiotic such as penicillin) to afford the aimed semifluid medium for diagnosis of trichomoniasis. |
