| 摘要 |
<p>The present invention provides a vector system for introduction of foreign DNA into a bacterial host by site-specific integration, characterized by utilization of phage øLC3 integration functions. More particularly, the invention provides a DNA molecule comprising the nucleotide sequence TTCTTCATG (SEQ ID NO. 1) serving as the core region of attP of phage øLC3 or of the corresponding attB site of a lactic acid bacterium host and a DNA molecule comprising a nucleotide sequence which acts as an integrase promoter and/or encodes an integrase and/or attP of phage øLC3, or a fragment thereof, substantially corresponding to all or a portion of the nucleotide sequence shown in Figure 3 (SEQ ID NO. 2) or a functionally equivalent sequence which is degenerate or substantially homologous with, or which hybridises with any such sequence, as well as vectors containing such DNA molecules.</p> |
