SLO EXPRESSION VECTOR, STRAIN TRANSFORMED WITH THE SAME VECTOR, PRODUCTION OF THE VECTOR AND SLO

摘要

PURPOSE:To obtain an SLO(streptolysin O) expression vector, having a larger production than that by the culture of Streptococcus pyogenes and capable of expressing the SLO, a transformed microorganism and a method for producing the SLO expression vector and SLO. CONSTITUTION:A DNA fragment, having the function of expression in a strain belonging to the genus Bacillus and containing a promoter of various genes derived from a strain belonging to the genus Bacillus or a DNA fragment containing the promoter and at least a pre-region is arranged on the downstream side of a mature slo structural gene to construct an SLO expression vector. A promoter derived from spoOA gene, alkaline protease gene and P-43 gene of Bacillus subtilis can be selected as the promoter. A pre-region derived from slo gene itself and alkaline protease can be selected as the pre-region. Thereby, SLO having the activity of about 3.5 to 32 times that produced by the original strain Streptococcus pyogenes is produced by investigating the combination and culture conditions. The figure illustrates the comparison of the hemolytic activity between the original strain Streptococcus pyogenes and a recombinant Bacillus subtilis.