Monoclonal antibody that distinguishes between phosphorylated and nonphosphorylated histone H1.

摘要

The present invention relates to a monoclonal antibody (mAb) that binds selectively to phosphorylated histone H1 and not to nonphosphorylated histone H1 and its use. In one embodiment, the mAb of the present invention binds selectively to histone H1 phosphorylated at the 12D11 epitope as defined herein. In a second aspect, the present invention relates to a cell producing a monoclonal antibody which binds selectively to phosphorylated histone H1 and has been shown to distinguish between histone H1 phosphorylated at the 12D11 epitope and histone H1 nonphosphorylated at this epitope. In another aspect, the present invention relates to a method for evaluating cells or tissue obtained from an individual for the presence of dedifferentiated (or nondifferentiated) cells. Kits are also the subject of the present invention and are suitable for practicing the method discussed above.