Method of spectrometry and apparatus therefor.

摘要

A method of spectrometry for measuring transient absorbance and transient absorption spectra of a substance to be measured at interfaces in the liquid phase of particulates containing a fluorescent pigment and the substance to be measured by the utilization of optical resonance phenomenon, and an apparatus therefor which comprises: (a) a pulse laser oscillator (2) for exciting the fluorescent pigment and the substance to be measured, respectively; (b) an optical delay device (3) for causing delay of any one of pulse laser beams of two kinds of wavelengths generated by said pulse laser oscillator; (c) a microscope system (4) for condensing laser beams generated by the pulse laser oscillator and irradiating the condensed beams to the sample; and (d) a detector (5) for detecting light emission of the sample. According to the method of the present invention, it is possible to measure transient absorbance and/or a transient absorption spectrum at a high sensitivity even when particulates containing the substance to be measured in the liquid phase are present. <IMAGE>