A HEPATITIS B VACCINE FORMULATION INCORPORATING A BILE ACID SALT

摘要

A novel, highly immunogenic hepatitis B vaccine formulation comprising HBsAg produced as the result of recombinant yeast host cell expression and a bile salt, particularly sodium deoxycholate, are disclosed, as are the methods used to produce the vaccine. These methods include novel yeast culture conditions and novel HBsAg purification and adjuvanting protocols. In addition, methods utilizing these novel HBsAg formulations to immunize mammals, particularly humans, susceptible to hepatitis B infection are also described.