| 摘要 |
PURPOSE:To provide a high-concentration and high-efficiency culture method for coli bacillus, capable of efficiently producing the objective substance in production of an enzyme, a bioactive substance, etc., by coli bacillus. CONSTITUTION:In relation to aerobic culture of coli bacillus, this high- concentration and high-efficiency culture method is carried out by adding an amino acid composition to the culture medium so that amino acids required for growth of the coli bacillus may not run short and simultaneously adding glucose to the medium so as to control pH of the medium to be a constant value in a state where pH of the medium increases. |
