| 摘要 |
PURPOSE:To provide a new tripeptide having a specific amino acid sequence, inhibiting angiotensin I converting enzyme, exhibiting hypotensive action and low side-action and useful as an agent for the treatment of hypertension, foods, etc. CONSTITUTION:A peptide is synthesized on a solid-phase supporting member by a solid-phase peptide synthesis process using Fmoc(9- fluorenylmethyloxycarbonyl)-amino acid, t-Boc(t-butoxycarbonyl)-amino acid, etc., the synthesized peptide is subjected to the removal from the solid phase and the elimination of the protecting groups and the product is treated with an anion exchange resin column and purified by reversed phase high-performance liquid chromatography to obtain the objective tripeptide effective for inhibiting angiotensin I converting enzyme and having the structure of Leu-Arg-(Trp, Phe, Thr), Ile-Arg-(Phe, Tyr), lie-Lys-Phe, Phe-Arg-(Pro, Trp, Phe, Tyr) and Phe-Lys-(Trp, Phe, Tyr). |
