| 摘要 |
The present invention concerns a mutant cephalosporin C acylase derived from a precursor of the formula: A1-268-X1-Tyr-X2-A272-304-X3-A306-773 (SEQ ID NO:1), wherein: A1-268 is the same amino acid sequence as that from Thr1 to Gly268 of native CC acylase, A272-304 is the same amino acid sequence as that from Gln272 to Tyr304 of native CC acylase, A306-773 is the same amino acid sequence as that from Val306 to Ala773 of native CC acylase, X1 is Met or other amino acid, X2 is Ala or Tyr, and X3 is Cys or Ser, provided that when X1 is Met and X2 is Ala, X3 is Ser; and that the mutant cephalosporin C acylase has a property selected from the group consisting of higher enzymatic potency and higher processing efficiency, as compared to native CC acylase. The present invention also concerns DNA encoding the mutant cephalosporin C acylase, an expression vector containing the DNA, a host cell transformed with the expression vector, a process for producing the mutant cephalosporin C acylase by culturing the transformed host cell, and a process for preparing a cephalosporin C using the mutant cephalosporin C acylase.
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